Fall 2005

1. Overview of molecular biology and cellular metabolism (3 lectures)

-         basic principles of life

-         the language of biology

-         transport processes

-         fueling reactions

-         biosynthetic reactions

-         polymerization

2. Modeling of metabolic networks (4 lectures)

-         stoichiometry

-         kinetics

-         mass balances for the steady state

-         mass balances for the transient case

3. Metabolic flux analysis (3 lectures)

-         linear programming

4. Methods for experimental determination of metabolic fluxes (1 lecture)

-         isotope labeling

5. Metabolic control analysis (3 lectures)

-         nonlinear programming

6. Synthesis and design of metabolic networks (3 lectures)

-         integer programming

-         mixed-integer nonlinear programming

7. Case studies (3 lectures)

-         ethanol production

-         amino acid biosynthesis

-         metabolism in bacteria and yeast

8. Paper presentations (3 lectures)

9. Project presentations (1 lecture)

10. Review (1 lecture)